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Effect Of Cholesterol Addition On Cryopreservation Of Spermatozoa And Field Fertility Of Nili Ravi Buffalo

by Abdul Razzaq | Dr. Mian Abdul Sattar | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1821,T] (1).

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Comparison Between Aspiration And Slicing Methods For Retrieval Of Oocytes In Bovine

by Muhammad Husnain (2008-VA-281) | Prof. Dr. Mian Abdul Sattar | Dr. Qaiser Shahzad | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Livestock contribution to agriculture stood at 55.9 percent while it contributes 11.8 percent to the national GDP during 2013-14. Buffalo, cattle, sheep and goat population in Pakistan is 34.6, 39.7, 29.1 and 66.6 million numbers during 2013-14. Total milk production from buffalo and cattle as major milk producing animals is 31,252 and 18,027 (000 tons) (Economic Survey of Pakistan 2013-14). Advanced biotechnologies coming from different areas of biological sciences exhibit great promise to enhance the efficiency of livestock production. From these technologies one such biotechnology is the use of in vitro maturation of follicular oocytes and in vitro fertilization for production of livestock embryos in laboratory. Proper oocytes recovery and their selection in the laboratory are of great importance for successful in vitro embryo production. Total one hundred and forty four ovaries (n=144) from cattle (72 ovaries) and buffalo (72 ovaries) were collected and 223 oocytes were retrieved from these ovaries. Average oocytes per ovary were 1.66 + 0.43 oocytes per ovary were obtained via aspiration and 1.89 + 0.00 average oocytes per ovary through slicing method from cattle ovaries. Average 1.55 ± 0.55 oocytes per ovary via aspiration and 1.53 ± 0.20 oocytes per ovary through slicing from buffalo ovaries. Overall grade-A oocytes were 28 (40) percent with aspiration in cattle and 25(36.76) through slicing method. In buffalo overall grade-A oocytes retrieval was obtained in percentage as 20 (44.44) and 26 (52) through aspiration and slicing methods respectively. Grade-B oocytes recovery obtained was in percentage as 23 (33.82) with slicing and 19 (31.67) through aspiration technique from cattle ovaries. Summary 26 Commonly used methods of recovery of oocytes from slaughterhouse animals are aspiration and slicing. Recovery rate of oocytes is different from slaughterhouse ovaries. Aspiration is the best method for retrieval of good quality oocytes from slaughterhouse bovine ovaries because it gave more good quality oocytes in less time than slicing method. In this study, it is found that weight of ovary and no. of follicles/ovary in cattle have strong correlation of 71% existed between weight of ovary and no. of follicles /ovary in buffalo was observed. Correlation between average number of follicles on ovary and weight /ovary was stronger in cattle. The more the number of follicles present on the ovaries and more weight of the ovary, the more will be the recovery of oocytes. In cattle average number of follicles was 10.09 ± 0.30 and when it was checked in buffalo, differed significantly and it was found as 7.16 ± 0.19 on an average per ovary. Likewise weight of buffalo in this study was differed significantly from cattle 4.04 ± 0.10 and 7.62 ± 0.15 respectively. It is suggested that oocytes retrieval should be done in buffalo using aspiration method to retrieve better quality oocytes. It is concluded that aspiration is the suitable method for retrieval of good quality oocytes from slaughterhouse buffalo ovaries because it gave more good quality oocytes in less time than slicing method. But both methods have minor difference between recovery rates but aspiration is more convenient than slicing and it yields more quality oocytes. It is also found that there is very strong correlation existed between average weight of ovary and number of follicles per ovary and the both parameters play a great help for more quality and quantity oocytes. Availability: Items available for loan: UVAS Library [Call number: 2279-T] (1).
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Effect Of Antibiotic Treatment During Ovulation Synchronization In Repeat Breeder Holstein Friesian Cows

by Masood Shabbir (2010-VA-120) | Dr. Muhammad Zahid Tahir | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Repeat breeding is one of the major causes of reproductive, productive and economic losses in dairy sector. The main causes of repeat breeding include sub-clinical infection of reproductive tract, age of the animal, error in the detection of estrus, endocrine dysfunction and nutritional deficiencies. In Pakistan, a very high incidence of repeat breeding has been documented (Kakar et al. 1997). In the past, intrauterine infusions have successfully been used with a variety of antiseptic and antibiotic solutions. Meanwhile, therapeutic use of GnRH and PGF2α has also been demonstrated to result in improved pregnancy rates. In particular, Ovsynch protocol leads to an increase of 6-17% in conception rate. The objective of the present study was to evaluate the reproductive efficiency with antibiotic treatment during ovulation synchronization in repeat breeder Holstein Friesian cows. This study was conducted on 30 pure bred Holstein Friesian cows kept under standard farm conditions of feeding and management at Hussain Cattle and Dairy Farm, Kasur. The reproductive efficiency of treated and control animals was based on ovulation rate, non-return rate, conception rate, pregnancy rate, embryonic losses and luteal function. Results were analyzed by independent T-test. A probability level of 95% was consider as significant (P<0.05). All the experimental animals were screened to confirm non-pregnant and normal genitalia. Both the treatment and control groups were synchronized using ovsynch protocol. Following first injection of GnRH, the treatment group was subjected to intrauterine antibiotic infusion for five days. On Day 7 of protocol both groups received an injection of PGF2α. At day 9 of ovsynch protocol before second injection of GnRH both groups were scanned for ovarian status. Follicles and CL measurement were noted and mapped by using 7.5 MHz trans-rectal probe (Honda 22 Summary Model HS-1600) in both groups and second injection of GnRH was given. Timed artificial insemination was performed after 16-20 hours of second injection of GnRH in both groups. After 8 hours of artificial insemination again ultrasonography was conducted to check the ovulation rate in both groups, there was no significant difference between control and treatment, 60% and 75% of the animals in control and treatment groups ovulate respectively. At day 11 blood samples were collected for progesterone assay. Non-return rate was visually observed at day 18 to 21 of artificial insemination. Blood was collected at day 18 for progesterone assay by puncture of tail vein. After D28 andD42 of artificial insemination first and second pregnancy test were conducted by using 7.5 MHz trans-rectal probe (Honda Model HS-1600) in control and treatment group. There was a significant difference of pregnancy among the control and treatment group by independent T-test. 3 rd and 4 th blood samples were collected for progesterone assay at day28 and 42 of artificial insemination in both groups respectively. Availability: Items available for loan: UVAS Library [Call number: 2951-T] (1).

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